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MAF1 antibody

KO/KD
Anti-MAF1 antibody used in Western Blot (WB). GTX106776
Anti-MAF1 antibody used in Western Blot (WB). GTX106776
Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776
Anti-MAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106776
Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776
Anti-MAF1 antibody used in Western Blot (WB). GTX106776
Anti-MAF1 antibody used in Western Blot (WB). GTX106776

Cat. No. GTX106776

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAF1 homolog, negative regulator of RNA polymerase III

Cellular Localization

Nucleus

Background

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

KO/KD

Wild-type (WT) and MAF1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody (GTX106776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody (GTX106776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776

GTX106776 IHC-P Image

MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MAF1 antibody (GTX106776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106776

GTX106776 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1(GTX106776) antibody at 1:200 dilution.

Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776

GTX106776 IHC-P Image

MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MAF1 antibody (GTX106776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

KO/KD

The data was published in the journal Elife in 2015.PMID: 26067234

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

KO/KD

The data was published in the journal Elife in 2015.PMID: 26067234

REFERENCE

Application Reference

Li Y et al. iScience 2023; 26 (12) : 108544 Maf1 controls retinal neuron number by both RNA Pol III- and Pol II-dependent mechanisms.
Lee HK et al. Sci Rep 2022; 12 (1) : 1646 Differential release of extracellular vesicle tRNA from oxidative stressed renal cells and ischemic kidneys.
Application : WB
Reactivity : Mouse
Lee YL et al. Elife 2015; 4 : e06283 MAF1 represses CDKN1A through a Pol III-dependent mechanism.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169