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MAF1 antibody

Anti-MAF1 antibody used in Western Blot (WB). GTX106776
Anti-MAF1 antibody used in Western Blot (WB). GTX106776
Anti-MAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106776
Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776
Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776

Cat No. GTX106776

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950848

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAF1 homolog, negative regulator of RNA polymerase III

Cellular Localization

Nucleus

Background

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

Wild-type (WT) and MAF1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody (GTX106776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAF1 antibody used in Western Blot (WB). GTX106776

GTX106776 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody (GTX106776) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-MAF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106776

GTX106776 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1(GTX106776) antibody at 1:200 dilution.

Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776

GTX106776 IHC-P Image

MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MAF1 antibody (GTX106776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106776

GTX106776 IHC-P Image

MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MAF1 antibody (GTX106776) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

MAF1 antibody Cat No. GTX106776

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26067234
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Elife in 2015.PMID: 26067234
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169