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MAFB antibody

Anti-MAFB antibody used in Western Blot (WB). GTX129437
Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437
Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

Cat No. GTX129437

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

MAFB-transfected 293T

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAF bZIP transcription factor B , DURS3 , KRML , MCTO

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAFB antibody used in Western Blot (WB). GTX129437

GTX129437 WB Image

MAFB antibody detects MAFB protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of MAFB-transfected 293T cells
10 % SDS-PAGE
MAFB antibody (GTX129437) dilution: 1:50000

Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

GTX129437 IHC-P Image

MAFB antibody detects MAFB protein at nucleus on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
MAFB antibody (GTX129437) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

GTX129437 IHC-P Image

MAFB antibody detects MAFB protein at nucleus on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
MAFB antibody (GTX129437) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf