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MAFB antibody

Anti-MAFB antibody used in Western Blot (WB). GTX129437
Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437
Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

Cat No. GTX129437

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all MAFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

MAFB-transfected 293T

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885995

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAF bZIP transcription factor B , DURS3 , KRML , MCTO

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAFB antibody used in Western Blot (WB). GTX129437

GTX129437 WB Image

MAFB antibody detects MAFB protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of MAFB-transfected 293T cells
10 % SDS-PAGE
MAFB antibody (GTX129437) dilution: 1:50000

Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

GTX129437 IHC-P Image

MAFB antibody detects MAFB protein at nucleus on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
MAFB antibody (GTX129437) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAFB antibody used in IHC (Paraffin sections) (IHC-P). GTX129437

GTX129437 IHC-P Image

MAFB antibody detects MAFB protein at nucleus on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
MAFB antibody (GTX129437) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169