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MAFF antibody

Orthogonal Overexpression
Anti-MAFF antibody used in Western Blot (WB). GTX120264
Anti-MAFF antibody used in Western Blot (WB). GTX120264
Anti-MAFF antibody used in IHC (Paraffin sections) (IHC-P). GTX120264
Anti-MAFF antibody used in Western Blot (WB). GTX120264
Anti-MAFF antibody used in Western Blot (WB). GTX120264

Cat. No. GTX120264

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAFF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Huh7 , HepG2 , human MAFF-transfected 293T , A431 nuclear extract , rat skin

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAFF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885476

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAF bZIP transcription factor F , U-MAF , hMafF

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAFF antibody used in Western Blot (WB). GTX120264

GTX120264 WB Image

Overexpression

MAFF antibody detects MAFF protein by western blot analysis. Non-transfected (-) and MAFF -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted by 1:20000.

Anti-MAFF antibody used in Western Blot (WB). GTX120264

GTX120264 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAFF antibody used in IHC (Paraffin sections) (IHC-P). GTX120264

GTX120264 IHC-P Image

MAFF antibody detects MAFF protein at cytoplasm and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MAFF antibody (GTX120264) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAFF antibody used in Western Blot (WB). GTX120264

GTX120264 WB Image

MAFF antibody detects MAFF protein by Western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
MAFF antibody (GTX120264) dilution: 1:2000

Anti-MAFF antibody used in Western Blot (WB). GTX120264

GTX120264 WB Image

Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX120264) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Moon EJ et al. Nat Commun 2021; 12 (1) : 4308 The HIF target MAFF promotes tumor invasion and metastasis through IL11 and STAT3 signaling.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169