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MAFF antibody

Orthogonal Overexpression
Anti-MAFF antibody used in Western Blot (WB). GTX129249
Anti-MAFF antibody used in Western Blot (WB). GTX129249

Cat. No. GTX129249

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAFF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

human MAFF-transfected 293T cells , A431 nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human MAFF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885941

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAF bZIP transcription factor F , U-MAF , hMafF

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAFF antibody used in Western Blot (WB). GTX129249

GTX129249 WB Image

Overexpression

MAFF antibody detects MAFF protein by western blot analysis.
293T mock transfected (-) or transfected with a construct expressing human MAFF(+).
12 % SDS-PAGE
MAFF antibody (GTX129249) dilution: 1:20000

Anti-MAFF antibody used in Western Blot (WB). GTX129249

GTX129249 WB Image

Orthogonal

A431 whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFF antibody (GTX129249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169