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MAFK antibody [AT2F7]

Anti-MAFK antibody [AT2F7] used in Western Blot (WB). GTX53738

Cat No. GTX53738

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2F7

Isotype

IgG2a

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human MAFK (1-156aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Maf Bzip Transcription Factor K , Nfe2U , P18 , Mafk

Background

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-MAFK antibody [AT2F7] used in Western Blot (WB). GTX53738

GTX53738 WB Image

WB analysis of Jurkat lysate (30ug) using MAFK antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf