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MAFK antibody

Anti-MAFK antibody used in ChIP assay (ChIP assay). GTX129240
Anti-MAFK antibody used in Immunoprecipitation (IP). GTX129240
Anti-MAFK antibody used in Western Blot (WB). GTX129240

Cat No. GTX129240

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ChIP assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human MAFK Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAF bZIP transcription factor K , NFE2U , P18

Cellular Localization

Nucleus

Background

The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-MAFK antibody used in ChIP assay (ChIP assay). GTX129240

GTX129240 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract treated with LPS (10 ng/ml for 1 h) and 5 μg of either normal rabbit IgG or anti-MAFK antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH .
ChIP experiment and primer designs are based on Sci Rep. 2013 Nov 19;3:3242.

Anti-MAFK antibody used in Immunoprecipitation (IP). GTX129240

GTX129240 IP Image

MAFK antibody immunoprecipitates MAFK protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of MAFK protein by 4 μg MAFK antibody (GTX129240)
15 % SDS-PAGE
The immunoprecipitated MAFK protein was detected by MAFK antibody (GTX129240) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-MAFK antibody used in Western Blot (WB). GTX129240

GTX129240 WB Image

MAFK antibody detects MAFK protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
15 % SDS-PAGE
MAFK antibody (GTX129240) dilution: 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf