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MAGE 1 antibody [N1C3]

Anti-MAGE 1 antibody [N1C3] used in Western Blot (WB). GTX103961
Anti-MAGE 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103961

Cat. No. GTX103961

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAGE 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAGE 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAGE family member A1 , CT1.1 , MAGE1

Cellular Localization

Cytoplasm

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAGE 1 antibody [N1C3] used in Western Blot (WB). GTX103961

GTX103961 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX103961 diluted at 1:1000

Anti-MAGE 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103961

GTX103961 ICC/IF Image

MAGE 1 antibody [N1C3] detects MAGE 1 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAGE 1 protein stained by MAGE 1 antibody [N1C3] (GTX103961) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169