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MAGEA11 antibody [N2N3]

Anti-MAGEA11 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110484
Anti-MAGEA11 antibody [N2N3] used in Western Blot (WB). GTX110484
Anti-MAGEA11 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX110484

Cat No. GTX110484

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , H1299
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MAGEA11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mage Family Member A11,Ct1.11,Mage-11,Mage11,Magea-11,Magea11

Cellular Localization

Nucleus , Cytoplasm

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-MAGEA11 antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110484

GTX110484 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAGEA11(GTX110484) antibody at 1:500 dilution.

Anti-MAGEA11 antibody [N2N3] used in Western Blot (WB). GTX110484

GTX110484 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
10% SDS PAGE
GTX110484 diluted at 1:5000

Anti-MAGEA11 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX110484

GTX110484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft , using MAGEA11(GTX110484) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
There are currently no references for MAGEA11 antibody [N2N3] (GTX110484).
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169