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MAGEA4 antibody

Orthogonal
Anti-MAGEA4 antibody used in Western Blot (WB). GTX116357
Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116357
Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116357
Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116357

Cat. No. GTX116357

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAGEA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431,U2OS

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAGEA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721176

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAGE family member A4 , CT1.4 , MAGE-41 , MAGE-X2 , MAGE4 , MAGE4A , MAGE4B

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAGEA4 antibody used in Western Blot (WB). GTX116357

GTX116357 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAGEA4 antibody (GTX116357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116357

GTX116357 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAGEA4(GTX116357) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116357

GTX116357 ICC/IF Image

MAGEA4 antibody detects MAGEA4 protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAGEA4 protein stained by MAGEA4 antibody (GTX116357) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116357

GTX116357 IHC-P Image

The data was published in the journal PLoS One in 2018.PMID: 29385186

REFERENCE

Application Reference

Eladak S et al. PLoS One 2018; 13 (1) : e0191934 Effects of environmental Bisphenol A exposures on germ cell development and Leydig cell function in the human fetal testis.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169