APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| Assay dependent |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Breast Tissue Slide(GTX24324) , A431 Cell Lysate(GTX27909)
Product Note
MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.The immunogen is located within the first 50 amino acids of MAGEA4.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
MAGE family member A4 , CT1.4 , MAGE-41 , MAGE-X2 , MAGE4 , MAGE4A , MAGE4B
Background
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31620 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31620 MAGEA4 antibody.
Working concentration : 5 μg/ml
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GTX31620 WB Image
WB analysis of A431 cell lysate using GTX31620 MAGEA4 antibody.
Working concentration : 1 μg/ml
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GTX31620 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml
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GTX31620 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml
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GTX31620 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml
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GTX31620 IHC-P Image
IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 5 μg/ml
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REFERENCE
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REVIEW
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