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MAGEA4 antibody

Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31620
Anti-MAGEA4 antibody used in Western Blot (WB). GTX31620
Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31620
Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620
Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620
Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620

Cat No. GTX31620

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ICC/IF Assay dependent
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Human Breast Tissue Slide(GTX24324) , A431 Cell Lysate(GTX27909)

Specificity/Sensitivity

MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.The immunogen is located within the first 50 amino acids of MAGEA4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

MAGE family member A4 , CT1.4 , MAGE-41 , MAGE-X2 , MAGE4 , MAGE4A , MAGE4B

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31620

GTX31620 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31620 MAGEA4 antibody.
Working concentration : 5 μg/ml

Anti-MAGEA4 antibody used in Western Blot (WB). GTX31620

GTX31620 WB Image

WB analysis of A431 cell lysate using GTX31620 MAGEA4 antibody.
Working concentration : 1 μg/ml

Anti-MAGEA4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31620

GTX31620 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml

Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620

GTX31620 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml

Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620

GTX31620 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 20 μg/ml

Anti-MAGEA4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31620

GTX31620 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX31620 MAGEA4 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339