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MAGED1 antibody

Orthogonal
Anti-MAGED1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00754
Anti-MAGED1 antibody used in Western Blot (WB). GTX00754
Anti-MAGED1 antibody used in Western Blot (WB). GTX00754
Anti-MAGED1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00754

Cat. No. GTX00754

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MAGED1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:500-1:1000
IHC-Fr 1:300
ELISA Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Recombinant MBT-fused mouse MAGE-D1 (aa 1-775 )

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

melanoma antigen, family D, 1 , 2810433C11Rik , 5430405L04Rik , DXBwg1492e , Dlixin , Dlxin-1 , Dlxin1 , MAGE-D1 , NRAGE

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Database

Research Area

DATA IMAGES

Anti-MAGED1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00754

GTX00754 ICC/IF Image

Orthogonal

ICC/IF analysis of C2C12 cells using GTX00754 MAGED1 antibody (D1) or MHC antibody (MHC). C2C12 myoblast cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). The arrowheads point to differentiated multinucleated myocytes. MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.

Anti-MAGED1 antibody used in Western Blot (WB). GTX00754

GTX00754 WB Image

WB analysis of various mouse embryo samples (E18.5) using GTX00754 MAGED1 antibody. Endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.
Loading : 20 μg

Anti-MAGED1 antibody used in Western Blot (WB). GTX00754

GTX00754 WB Image

WB analysis of the cerebral cortex from mouse embryo (E16.5) using GTX00754 MAGED1 antibody.
Dilution : 1:3000
Loading : 10 μg

Anti-MAGED1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00754

GTX00754 IHC-Fr Image

IHC-Fr analysis of mouse embryos using GTX00754 MAGED1 antibody.
The arrowheads indicate the ventricular proliferative zone; V : Ventricle; C : Bone Cavity. MAGE-D1 was concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.
Upper : E12.5
Lower : E14.5
Dilution : 1:300

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399