APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:3000 |
1:500-1:1000 |
1:300 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Mouse
Immunogen
Recombinant MBT-fused mouse MAGE-D1 (aa 1-775 )
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
melanoma antigen, family D, 1 , 2810433C11Rik , 5430405L04Rik , DXBwg1492e , Dlixin , Dlxin-1 , Dlxin1 , MAGE-D1 , NRAGE
Cellular Localization
Nucleus,Cytoplasm,Cell membrane
Database
Research Area
DATA IMAGES
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GTX00754 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX00754 MAGED1 antibody (D1) or MHC antibody (MHC). C2C12 myoblast cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). The arrowheads point to differentiated multinucleated myocytes. MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.
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GTX00754 WB Image
WB analysis of various mouse embryo samples (E18.5) using GTX00754 MAGED1 antibody. Endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner. Loading : 20 μg
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GTX00754 WB Image
WB analysis of the cerebral cortex from mouse embryo (E16.5) using GTX00754 MAGED1 antibody. Dilution : 1:3000 Loading : 10 μg
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GTX00754 IHC-Fr Image
IHC-Fr analysis of mouse embryos using GTX00754 MAGED1 antibody. The arrowheads indicate the ventricular proliferative zone; V : Ventricle; C : Bone Cavity. MAGE-D1 was concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone. Upper : E12.5 Lower : E14.5 Dilution : 1:300
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REFERENCE
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REVIEW
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