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MAGEG1 antibody

Anti-MAGEG1 antibody used in Western Blot (WB). GTX00755
Anti-MAGEG1 antibody used in Western Blot (WB). GTX00755
Anti-MAGEG1 antibody used in Immunoprecipitation (IP). GTX00755

Cat No. GTX00755

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA, Purification

Reactivity

Human, Mouse, Rat
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:300-1:1000
IP Assay dependent
ELISA Assay dependent
Purification Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Recombinant MBT-fused mouse MAGE-G1 (aa 1-279)

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NSE3 homolog, SMC5-SMC6 complex component , 5730494G16Rik , AI642138 , BB044375 , HCA4 , Mageg1 , Ndnl2 , mage-g1

Cellular Localization

Cytoplasm,Nucleus

Database

DATA IMAGES

Anti-MAGEG1 antibody used in Western Blot (WB). GTX00755

GTX00755 WB Image

WB analysis of P19 cells at different stages of neural differentiation using GTX00755 MAGEG1 antibody. The result revealed that P19 cells express MAGE-G1 (32 kDa) during the course of neuronal differentiation. The level of MAGE-G1 was the highest in retinoic acid-treated P19 cells.
UN : Undifferentiated P19 cells
RA : Aggregated cells treated with retinoic acid
PN : Enriched postmitotic neurons

Anti-MAGEG1 antibody used in Western Blot (WB). GTX00755

GTX00755 WB Image

WB analysis of mouse embryonic fibroblast (E14.5) using GTX00755 MAGEG1 antibody.
Dilution : 1:300
Loading : 10 μg

Anti-MAGEG1 antibody used in Immunoprecipitation (IP). GTX00755

GTX00755 IP Image

IP analysis of P19 cell lysate using GTX00755 MAGEG1 antibody.
The lysate from retinoic acid-treated P19 cells was applied to immunoaffinity columns of anti-MAGE-G1 IgG (alpha G1 IgG) and preimmune IgG (Preimmune IgG). Bound proteins were immunoblotted for E2F1, p75NTR, and MAGE-G1 (G1) with respective antibodies. MAGE-G1 endogenously forms stable complexes with E2F1 and p75NTR in differentiated P19 cells.

REFERENCE

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Package List Price ($)
$ 359