WB analysis of P19 cells at different stages of neural differentiation using GTX00755 MAGEG1 antibody. The result revealed that P19 cells express MAGE-G1 (32 kDa) during the course of neuronal differentiation. The level of MAGE-G1 was the highest in retinoic acid-treated P19 cells.
UN : Undifferentiated P19 cells
RA : Aggregated cells treated with retinoic acid
PN : Enriched postmitotic neurons

IP analysis of P19 cell lysate using GTX00755 MAGEG1 antibody.
The lysate from retinoic acid-treated P19 cells was applied to immunoaffinity columns of anti-MAGE-G1 IgG (alpha G1 IgG) and preimmune IgG (Preimmune IgG). Bound proteins were immunoblotted for E2F1, p75NTR, and MAGE-G1 (G1) with respective antibodies. MAGE-G1 endogenously forms stable complexes with E2F1 and p75NTR in differentiated P19 cells.

WB analysis of mouse embryonic fibroblast (E14.5) using GTX00755 MAGEG1 antibody.
Dilution : 1:300
Loading : 10 μg