GeneTex
United States (US)

MAGI3 antibody

Cat No. GTX11511

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1.0 μg/ml
ICC/IF 2.0 μg/ml

Note :

WB
using enzyme-treated human tissue sections

Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Specificity/Sensitivity

Staining by immunoblotting and immunofluorescence is specifically inhibited by the immunizing peptide.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% bovine serum albumin, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

synthetic peptide corresponding to amino acids 835-850 of SLIPR (rat homolog of MAGI-3) conjugated to KLH via a N-terminal added lysine residue.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Membrane Associated Guanylate Kinase, Ww And Pdz Domain Containing 3,Slipr,Magi3

Background

MAGI3 (membrane-associated guanylate cyclase of inverted orientation-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs) that comprise peripheral membrane proteins involved in the formation of specialized cell-cell junctions. MAGI3, as well as other proteins in this family are characterized by three types of protein-protein interaction modules: five PDZ domains, an N-terminal guanylate kinase (GK) domain, and a WW domain. The WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, mediates protein-protein interactions through binding to short proline-rich sequences). MAGI3 and PTEN/MMAC together modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions PTEN/MMAC phosphatase to specific subcellular locations that are involved in the regulation of cell proliferation and survival.

Database

Research Area

Package List Price ($)
$ 329