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MAK antibody [N1N3]

Anti-MAK antibody [N1N3] used in Western Blot (WB). GTX107556
Anti-MAK antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX107556
Anti-MAK antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX107556

Cat. No. GTX107556

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all MAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950852

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

male germ cell associated kinase , RP62

Background

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAK antibody [N1N3] used in Western Blot (WB). GTX107556

GTX107556 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
7.5% SDS PAGE
GTX107556 diluted at 1:1000

Anti-MAK antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX107556

GTX107556 IHC-Wm Image

MAK antibody [N1N3] detects Mak protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
MAK antibody [N1N3] (GTX107556) dilution: 1:200.
Anti-MAK antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX107556

GTX107556 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using MAK antibody [N1N3] (GTX107556) at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169