GeneTex
United States (US)

MAML1 antibody, N-term

GTX17019_392_IHC-P_18121410_697.jpg
GTX17019_1408_WB_18121410_256.jpg

Cat No. GTX17019

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 2.5-3.8μg/ml

Note :

IHC-P
Human Placenta shows nuclear staining in trophoblasts.

Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17019-PEP).

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence VLPTCPMAEFALPR, from the N Terminus of the protein sequence according to NP_055572.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mastermind Like Transcriptional Coactivator 1,Mam-1,Mam1,Maml1

Cellular Localization

Nuclear, in a punctate manner.

Background

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17019_392_IHC-P_18121410_697.jpg

GTX17019 IHC-P Image

IHC-P analysis of human placenta using GTX17019 MAML1 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX17019_1408_WB_18121410_256.jpg

GTX17019 WB Image

WB analysis of human skeletal muscle lysate using GTX17019 MAML1 antibody, N-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 319