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MAML1 antibody [N1], N-term

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748
Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748
Anti-MAML1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104748

Cat No. GTX104748

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all MAML1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus , mouse heart

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MAML1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mastermind like transcriptional coactivator 1 , Mam-1 , Mam1

Cellular Localization

Nucleus speckle

Background

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748

GTX104748 WB Image

Sample (30 ug of whole cell lysate)

A: HeLa

B: HeLa nucleus

7.5% SDS PAGE

GTX104748 diluted at 1:1000

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748

GTX104748 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX104748 diluted at 1:1000

Anti-MAML1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104748

GTX104748 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MAML1(GTX104748) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169