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MAML1 antibody [N1], N-term

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748
Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748
Anti-MAML1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104748

Cat No. GTX104748

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all MAML1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus , mouse heart

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MAML1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241038

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mastermind like transcriptional coactivator 1 , Mam-1 , Mam1

Cellular Localization

Nucleus speckle

Background

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748

GTX104748 WB Image

Sample (30 ug of whole cell lysate)

A: HeLa

B: HeLa nucleus

7.5% SDS PAGE

GTX104748 diluted at 1:1000

Anti-MAML1 antibody [N1], N-term used in Western Blot (WB). GTX104748

GTX104748 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX104748 diluted at 1:1000

Anti-MAML1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104748

GTX104748 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MAML1(GTX104748) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169