MAML2 antibody

Cat No. GTX131250

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human
Package	100 μl ($329), 25 μl ($169)

See all MAML2 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAML2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886450

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mastermind like transcriptional coactivator 2 , MAM-3 , MAM2 , MAM3 , MLL-MAML2

Cellular Localization

Nucleus speckle

Background

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.

Database

Gene ID: 84441 MAML2
UniProt: Q8IZL2 MAML2

Research Area

DATA IMAGES

GTX131250 WB Image

MAML2 antibody detects MAML2 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAML2 antibody (GTX131250) diluted at 1:500.

GTX131250 IP Image

Immunoprecipitation of MAML2 protein from HeLa whole cell extracts using 5 μg of MAML2 antibody (GTX131250).
Western blot analysis was performed using MAML2 antibody (GTX131250) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 329

25 μl

$ 169

Qty