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MAN1B1 antibody [3C2]

KO/KD
Anti-MAN1B1 antibody [3C2] used in IHC (Paraffin sections) (IHC-P). GTX03413
Anti-MAN1B1 antibody [3C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03413
Anti-MAN1B1 antibody [3C2] used in Western Blot (WB). GTX03413

Cat. No. GTX03413

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human
Package
100 μl ($449)
See all MAN1B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100
IHC-P 1:200
IP 1:10-1:100
ELISA 1:1000

Note :

IHC-P
Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse samples.
Knockdown/Knockout validation was supported by references (PMID: 26205822).

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine pH 7.5, 0.15M NaCl

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant ERManI [Swiss-Prot# Q9UKM7].

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannosidase alpha class 1B member 1 , ERMAN1 , ERManI , MANA-ER , MRT15

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-MAN1B1 antibody [3C2] used in IHC (Paraffin sections) (IHC-P). GTX03413

GTX03413 IHC-P Image

IHC-P analysis of human breast cancer using GTX03413 MAN1B1 antibody [3C2].

Anti-MAN1B1 antibody [3C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03413

GTX03413 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03413 MAN1B1 antibody [3C2].
Green : Primary antibody
Red : alpha tubulin
Blue : DAPI

Anti-MAN1B1 antibody [3C2] used in Western Blot (WB). GTX03413

GTX03413 WB Image

WB analysis of various sample lysates using GTX03413 MAN1B1 antibody [3C2].
Lane 1 : NTERA-2 whole cell lysate
Lane 2 : A431 whole cell lysate

REFERENCE

Clone Reference

T Zhou et al. et al. J Biol Chem 2015; 290 (36):22184–22192 ERManI (Endoplasmic Reticulum Class I α-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 449