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MAN1B1 antibody [C1C3]

Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882
Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882
Anti-MAN1B1 antibody [C1C3] used in Western Blot (WB). GTX105882
Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882

Cat No. GTX105882

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAN1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mannosidase Alpha Class 1B Member 1,Erman1,Ermani,Mana-Er,Mrt15,Man1B1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein

Background

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2).

Database

DATA IMAGES
Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882

GTX105882 IHC-P Image

MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
MAN1B1 antibody [C1C3] (GTX105882) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882

GTX105882 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC25 xenograft, using MAN1B1(GTX105882) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAN1B1 antibody [C1C3] used in Western Blot (WB). GTX105882

GTX105882 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
MAN1B1 antibody
GTX105882 diluted at 1:1000

Anti-MAN1B1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105882

GTX105882 IHC-P Image

MAN1B1 antibody [C1C3] detects MAN1B1 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
MAN1B1 antibody [C1C3] (GTX105882) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169