GeneTex

  • Country / Location Selection

United States (US)

MANEA antibody, Internal

Anti-MANEA antibody, Internal used in Western Blot (WB). GTX45981
Anti-MANEA antibody, Internal used in Western Blot (WB). GTX45981

Cat. No. GTX45981

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($399)
See all MANEA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , human fetal heart

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human MANEA

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mannosidase endo-alpha , ENDO , hEndo

Cellular Localization

Golgi apparatus membrane

Background

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-MANEA antibody, Internal used in Western Blot (WB). GTX45981

GTX45981 WB Image

WB analysis of human fetal heart tissue using GTX45981 MANEA antibody at 1μg/ml.

Anti-MANEA antibody, Internal used in Western Blot (WB). GTX45981

GTX45981 WB Image

WB analysis of 293T cells using GTX45981 MANEA antibody at 0.2-1μg/ml.

REFERENCE

There are currently no references for MANEA antibody, Internal (GTX45981). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for MANEA antibody, Internal (GTX45981). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399