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MAP1B antibody

Anti-MAP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133209
Anti-MAP1B antibody used in Western Blot (WB). GTX133209
Anti-MAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX133209
Anti-MAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX133209

Cat No. GTX133209

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Positive Control

SK-N-AS , C8D30
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

microtubule-associated protein 1B,A230055D22,AI843217,LC1,MAP5,Mtap-5,Mtap1b,Mtap5

Background

Attaches integral membrane proteins to cytoskeletal elements. Also binds to cytoskeletal proteins. Required for coordinate assembly of Na/Ca exchanger, Na/K ATPase and InsP3 receptor at sarcoplasmic reticulum sites in cardiomyocytes. Required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) in the inner segment of rod photoreceptors. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate.

Database

Research Area

DATA IMAGES
Anti-MAP1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133209

GTX133209 ICC/IF Image

MAP1B antibody detects MAP1B protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP1B protein stained by MAP1B antibody (GTX133209) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP1B antibody used in Western Blot (WB). GTX133209

GTX133209 WB Image

Various whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP1B antibody (GTX133209) diluted at 1:2000.

Anti-MAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX133209

GTX133209 IHC-P Image

MAP1B antibody detects MAP1B protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP1B antibody (GTX133209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP1B antibody used in IHC (Paraffin sections) (IHC-P). GTX133209

GTX133209 IHC-P Image

MAP1B antibody detects MAP1B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MAP1B antibody (GTX133209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169