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MAP2 antibody [AP-20]

Anti-MAP2 antibody [AP-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11268
Anti-MAP2 antibody [AP-20] used in Western Blot (WB). GTX11268
Anti-MAP2 antibody [AP-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11268

Cat. No. GTX11268

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AP-20

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine, Quail, Xenopus, aquatic salamander
Package
50 μl ($399)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Positive Control

rat cerebral cortex

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

bovine MAP2

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_369978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 2

Background

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody [AP-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11268

GTX11268 ICC/IF Image

ICC/IF analysis of B35 cells using GTX11268 MAP2 antibody [AP-20] at 1:100(red) with DAPI(blue).Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-MAP2 antibody [AP-20] used in Western Blot (WB). GTX11268

GTX11268 WB Image

WB analysis of rat brain extract using GTX11268 MAP2 antibody[AP-20].
Lane A : Antibody dilution 1:500
Lane B : Secondary antibody only

Anti-MAP2 antibody [AP-20] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11268

GTX11268 ICC/IF Image

ICC/IF analysis of SH-SY-5Y cells using GTX11268 MAP2 antibody [AP-20] at 1:500(red) with DAPI(blue).Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE

Application Reference

Valle-Bautista R et al. Front Cell Dev Biol 2020; 8 : 564561 Impaired Cortical Cytoarchitecture and Reduced Excitability of Deep-Layer Neurons in the Offspring of Diabetic Rats.
Reactivity : Rat
Berenice M?rquez-Valadez et al. Front. Neurosci. 2019; (Epub) The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist.
Application : IHC-P
Reactivity : Rat
Tian E et al. Cell Rep 2016; 16 (3) : 781-92 Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399