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MAP2 antibody [GT925]

Anti-MAP2 antibody [GT925] used in IHC (Paraffin sections) (IHC-P). GTX634473
Anti-MAP2 antibody [GT925] used in Western Blot (WB). GTX634473
Anti-MAP2 antibody [GT925] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634473

Cat. No. GTX634473

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT925

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888458

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody [GT925] used in IHC (Paraffin sections) (IHC-P). GTX634473

GTX634473 IHC-P Image

MAP2 antibody [GT925] detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
MAP2 stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody [GT925] used in Western Blot (WB). GTX634473

GTX634473 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody [GT925] (GTX634473) diluted at 1:6000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-MAP2 antibody [GT925] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634473

GTX634473 ICC/IF Image

MAP2 antibody [GT925] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dendrite marker MAP2 stained by MAP2 antibody [GT925] (GTX634473) diluted at 1:500.
Red: Axon marker Tau, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Hou B et al. Cell Death Dis 2020; 11 (5) : 377 Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations