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MAP2 antibody [HL1655]

Recombinant antibody
Anti-MAP2 antibody [HL1655] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637253
Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253
Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253
Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253

Cat. No. GTX637253

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1655

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Background

Enables microtubule binding activity. Involved in microtubule cytoskeleton organization; negative regulation of axon extension; and regulation of protein localization. Acts upstream of or within several processes, including establishment of cell polarity; microtubule bundle formation; and neuron projection development. Located in several cellular components, including axon; dendrite; and proximal neuron projection. Is expressed in several structures, including back skin; central nervous system; peripheral nervous system ganglion; retina; and trigeminal nerve. Orthologous to human MAP2 (microtubule associated protein 2). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody [HL1655] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637253

GTX637253 ICC/IF Image

MAP2 antibody [HL1655] detects MAP2 protein in dendrite/axon, by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253

GTX637253 IHC-P Image

MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253

GTX637253 IHC-P Image

MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody [HL1655] used in IHC (Paraffin sections) (IHC-P). GTX637253

GTX637253 IHC-P Image

MAP2 antibody [HL1655] detects MAP2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
MAP2 stained by MAP2 antibody [HL1655] (GTX637253) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations