MAP2 antibody [HM-2]

Cat. No. GTX11267

Reference 11

Host	Mouse
Clonality	Monoclonal
Clone Name	HM-2
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity	Human, Mouse, Rat, Bovine, Chicken, Quail
Package	50 μl ($399)

See all MAP2 products

View product citations for antibody GTX11267 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC	Assay dependent

Not tested in other applications.

Calculated MW

202 kDa. ( Note )

Positive Control

rat brain

Product Note

Monoclonal Anti-MAP2 antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

rat brain microtubule-associated proteins (MAPs).

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_369977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule-associated protein 2 , MAP2R , Mtap2

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Background

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

Database

Gene ID: 25595 Map2
UniProt: P15146 Map2

Research Area

DATA IMAGES

GTX11267 IHC-Fr Image

MAP2 antibody [HM-2] detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:500.

GTX11267 IHC-P Image

MAP2 antibody [HM-2] detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 antibody [HM-2] (GTX11267) diluted at 1:500.

GTX11267 ICC/IF Image

MAP2 antibody [HM-2] detects MAP2 protein at by immunofluorescent analysis.
Sample: Rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.

GTX11267 IHC-P Image

MAP2 antibody [HM-2] detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MAP2 antibody [HM-2] (GTX11267) diluted at 1:500.

GTX11267 IHC-Fr Image

MAP2 antibody [HM-2] detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX11267 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP2 antibody [HM-2] (GTX11267) diluted at 1:2000. The signal was developed with Trident ECL plus-Enhanced.

GTX11267 WB Image

WB analysis of rat brain extract using GTX11267 MAP2 antibody [HM-2]at 1 μg/mL.

GTX11267 ICC/IF Image

ICC/IF analysis of B35 cells using GTX11267 MAP2 antibody [HM-2] at 2 μg/mL(red) with DAPI(blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.