GeneTex
United States (US)

MAP2 antibody [MT-01]

MAP2-antibody-MT-01__GTX27756-1_18121411_871.jpg

Cat No. GTX27756

Host Mouse
Clonality Monoclonal
Clone Name MT-01
Isotype IgG1
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat, Bovine, Cat, Pig
APPLICATION

Application Note

ICC: Use at an assay dependant concentration. IP: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Thermostable fraction of bovine brain microtubules.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein 2,Map2

Cellular Localization

Cytoplasmic

Background

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.

Research Area

DATA IMAGES
MAP2-antibody-MT-01__GTX27756-1_18121411_871.jpg

GTX27756 WB Image

Western Blot analysis (reducing conditions) of microtubules partially purified from porcine brain lysate.
Lane 1: immunostaining with anti-MAP2ab (MT-01) (GTX27756)
Lane 2: immunostaining with anti-MAP2ab (MT-07) (GTX79912 )
Lane 3: immunostaining with anti-MAP2ab (MT-08) (GTX79911)

REFERENCE
Package List Price ($)
$ 319