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MAP2 antibody

Anti-MAP2 antibody used in Western Blot (WB). GTX133109
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

Cat. No. GTX133109

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Fish
Package
100 μl ($399),
25 μl ($169)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886830

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody used in Western Blot (WB). GTX133109

GTX133109 WB Image

Mouse tissue extract (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GTX133109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis .
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Grenn: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red (Top right): Dendrites, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Red (Bottom right): Axons, stained by Tau antibody (GTX49353 ) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133109

GTX133109 IHC-Fr Image

MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:250.
Red: Tau, stained by Tau antibody (GTX49353) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Wu SH et al. Biomedicines 2021; 9 (11) Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells.
Application : ICC/IF
Reactivity : Human
Wu SH et al. Front Cell Dev Biol 2021; 9 : 658099 Adipose-Derived Mesenchymal Stem Cells From a Hypoxic Culture Improve Neuronal Differentiation and Nerve Repair.
Application : ICC/IF
Reactivity : Human
Wang JP et al. J Tissue Eng Regen Med 2020; 14 (12) : 1804-1814 Mesenchymal stem cells from a hypoxic culture improve nerve regeneration.
Application : ICC/IF
Reactivity : Human
Wang L et al. J Cereb Blood Flow Metab 2020; : 271678X20952264 Remote ischemic conditioning enhances oxygen supply to ischemic brain tissue in a mouse model of stroke: Role of elevated 2,3-biphosphoglycerate in erythrocytes.
Reactivity : Mouse
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106 : 161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
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REVIEW

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Application Reference
Wu SH et al. Biomedicines 2021; 9 (11) Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells.
Application : ICC/IF
Reactivity : Human
Wu SH et al. Front Cell Dev Biol 2021; 9:658099 Adipose-Derived Mesenchymal Stem Cells From a Hypoxic Culture Improve Neuronal Differentiation and Nerve Repair.
Application : ICC/IF
Reactivity : Human
Wang JP et al. J Tissue Eng Regen Med 2020; 14 (12):1804-1814 Mesenchymal stem cells from a hypoxic culture improve nerve regeneration.
Application : ICC/IF
Reactivity : Human
Wang L et al. J Cereb Blood Flow Metab 2020;:271678X20952264 Remote ischemic conditioning enhances oxygen supply to ischemic brain tissue in a mouse model of stroke: Role of elevated 2,3-biphosphoglycerate in erythrocytes.
Reactivity : Mouse
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106:161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
Zhang J et al. Front Cell Neurosci 2020; 14:195 Mouse Astrocytes Promote Microglial Ramification by Releasing TGF-β and Forming Glial Fibers.
Ruiz-Palacios M et al. Sci Total Environ 2020; 708:134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.
Application : ICC/IF
Nan H et al. J Hum Genet 2019; 64 (11):1055-1065 UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes.
Application : ICC/IF
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169