MAP2 antibody

Name: MAP2 antibody
Brand: GeneTex
SKU: GTX133109
Price: 329.00 USD

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109

Anti-MAP2 antibody used in Western Blot (WB). GTX133109

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133109

Cat No. GTX133109

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain , DIV9 rat E18 primary cortical neurons

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Mouse MAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Gene ID: 17756 Map2
UniProt: P20357 Map2

Research Area

DATA IMAGES

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red: Tau, stained by Tau antibody (GTX49353 ) diluted at 1:500.
Blue:Fluoroshield with DAPI (GTX30920).

GTX133109 WB Image

Mouse tissue extract (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GTX133109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis .
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Grenn: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red (Top right): Dendrites, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Red (Bottom right): Axons, stained by Tau antibody (GTX49353 ) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133109 IHC-Fr Image

MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:250.
Red: Tau, stained by Tau antibody (GTX49353) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:500 and 1:5000.
Red: Axon marker Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133109 IHC-P Image

REFERENCE

Application Reference

Ruiz-Palacios M et al. Sci Total Environ 2020; 708 : 134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.

Application : ICC/IF

Nan H et al. J Hum Genet 2019; 64 (11) : 1055-1065 UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes.

Application : ICC/IF

Reactivity : Mouse

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REVIEW

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Application Reference

Ruiz-Palacios M et al. Sci Total Environ 2020; 708:134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.

Application : ICC/IF

Nan H et al. J Hum Genet 2019; 64 (11):1055-1065 UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes.

Application : ICC/IF

Reactivity : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 329
25 μl	$ 169
Qty

MAP2 antibody

Cat No. GTX133109

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX133109 ICC/IF Image

GTX133109 WB Image

GTX133109 ICC/IF Image

GTX133109 IHC-P Image

GTX133109 IHC-P Image

GTX133109 ICC/IF Image

GTX133109 IHC-Fr Image

GTX133109 IHC-P Image

GTX133109 IHC-P Image

GTX133109 IHC-P Image

GTX133109 ICC/IF Image

GTX133109 IHC-P Image

GTX133109 IHC-P Image

REFERENCE

Application Reference

REVIEW

Pamphlet - Recombinant Active Proteins

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