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MAP2 antibody

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30663
Anti-MAP2 antibody used in Western Blot (WB). GTX30663

Cat. No. GTX30663

Reference 2

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat, Bovine
Package
50 μl ($649)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:10000 - 1:20000
ICC/IF 1:5000 - 1:10000
IHC-Fr 1:1000 - 1:5000
IHC 1:1000 - 1:5000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

5mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified MAP2 isolated from bovine brain.

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

RRID

AB_625961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons.

Research Area

DATA IMAGES

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30663

GTX30663 ICC/IF Image

ICC/IF analysis of mixed neuron/glia cell cultures using GTX30663 MAP2 antibody. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody, while the axons of the neurons and the processes of all other cell types in these cultures (astrocytes, oligodendrocytes, microglia, endothelia and fibroblasts) are all negative.

Anti-MAP2 antibody used in Western Blot (WB). GTX30663

GTX30663 WB Image

WB analysis of bovine brain tissue lysate using GTX30663 MAP2 antibody.

REFERENCE

Application Reference

Goldman JS et al. J Neurosci 2013; 33 (44) : 17278-89 Netrin-1 promotes excitatory synaptogenesis between cortical neurons by initiating synapse assembly.
Application : ICC/IF
Reactivity : Mouse
Zagami CJ et al. PLoS One 2010; 5 (11) : e13944 Molecular characterization of the mouse superior lateral parabrachial nucleus through expression of the transcription factor Runx1.
Application : ICC/IF
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 649