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MAP2 antibody

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85455
Anti-MAP2 antibody used in Immunohistochemistry (IHC). GTX85455

Cat. No. GTX85455

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
ICC/IF Assay dependent
IHC 1:1000-1:2000
Not tested in other applications.

Calculated MW

200 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 2 , MAP-2 , MAP2A , MAP2B , MAP2C

Cellular Localization

Cytoplasm, cytoskeleton , Cell projection, dendrite

Background

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85455

GTX85455 ICC/IF Image

IHC analysis of E18 mouse brain using GTX85455 MAP2 antibody.
Green : Primary antibody
Blue : DAPI

Anti-MAP2 antibody used in Immunohistochemistry (IHC). GTX85455

GTX85455 IHC Image

IHC analysis of neonatal mouse brain cortical cultures using GTX85455 MAP2 antibody.
Green : Primary antibody
Red : GFAP antibody
Blue : DAPI

REFERENCE

Application Reference

Bassil R et al. Nat Commun 2021; 12 (1) : 5220 Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399