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MAP3K1 (phospho Thr1400) antibody

Anti-MAP3K1 (phospho Thr1400) antibody used in Western Blot (WB). GTX55333
Anti-MAP3K1 (phospho Thr1400) antibody used in IHC (Paraffin sections) (IHC-P). GTX55333

Cat. No. GTX55333

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all MAP3K1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 1400 (K-G-T(p)-G-A) derived from human MAP3K1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 1 , MAPKKK1 , MEKK , MEKK 1 , MEKK1 , SRXY6

Background

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-MAP3K1 (phospho Thr1400) antibody used in Western Blot (WB). GTX55333

GTX55333 WB Image

WB analysis of extracts from JK cells (lane 2) using GTX55333 MAP3K1 (phospho Thr1400) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

Anti-MAP3K1 (phospho Thr1400) antibody used in IHC (Paraffin sections) (IHC-P). GTX55333

GTX55333 IHC-P Image

IHC-P analysis of human brain tissue using GTX55333 MAP3K1 (phospho Thr1400) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399