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MAP3K1 (phospho Thr1400) antibody

Anti-MAP3K1 (phospho Thr1400) antibody used in Western Blot (WB). GTX55333
Anti-MAP3K1 (phospho Thr1400) antibody used in IHC (Paraffin sections) (IHC-P). GTX55333

Cat No. GTX55333

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 1400 (K-G-T(p)-G-A) derived from human MAP3K1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 1 , MAPKKK1 , MEKK , MEKK 1 , MEKK1 , SRXY6

Background

The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-MAP3K1 (phospho Thr1400) antibody used in Western Blot (WB). GTX55333

GTX55333 WB Image

WB analysis of extracts from JK cells (lane 2) using GTX55333 MAP3K1 (phospho Thr1400) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

Anti-MAP3K1 (phospho Thr1400) antibody used in IHC (Paraffin sections) (IHC-P). GTX55333

GTX55333 IHC-P Image

IHC-P analysis of human brain tissue using GTX55333 MAP3K1 (phospho Thr1400) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

There are currently no reviews for MAP3K1 (phospho Thr1400) antibody (GTX55333). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339