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MAP3K13 antibody

Anti-MAP3K13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111101
Anti-MAP3K13 antibody used in Western Blot (WB). GTX111101

Cat No. GTX111101

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

AsPC1

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885297

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 13 , LZK , MEKK13 , MLK

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MAP3K13 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111101

GTX111101 ICC/IF Image

MAP3K13 antibody detects MAP3K13 protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: MAP3K13 protein stained by MAP3K13 antibody (GTX111101) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-MAP3K13 antibody used in Western Blot (WB). GTX111101

GTX111101 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K13 antibody (GTX111101) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169