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MAP3K14 antibody

Anti-MAP3K14 antibody used in IHC (Paraffin sections) (IHC-P). GTX109419
Anti-MAP3K14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109419

Cat No. GTX109419

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K14. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885274

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 14 , FTDCR1B , HS , HSNIK , NIK

Cellular Localization

Cytoplasm

Background

This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MAP3K14 antibody used in IHC (Paraffin sections) (IHC-P). GTX109419

GTX109419 IHC-P Image

MAP3K14 antibody detects MAP3K14 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
MAP3K14 antibody (GTX109419) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAP3K14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109419

GTX109419 ICC/IF Image

MAP3K14 antibody detects MAP3K14 protein at cytoplasm by immunofluorescent analysis.
Sample: K562 cells were fixed in ice-cold MeOH for 5 min.
Green: MAP3K14 protein stained by MAP3K14 antibody (GTX109419) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169