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MAP3K8 antibody [HL2118]

Recombinant antibody Orthogonal Overexpression
Anti-MAP3K8 antibody [HL2118] used in IHC (Paraffin sections) (IHC-P). GTX638090
Anti-MAP3K8 antibody [HL2118] used in Western Blot (WB). GTX638090
Anti-MAP3K8 antibody [HL2118] used in Western Blot (WB). GTX638090

Cat. No. GTX638090

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2118

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MAP3K8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human MAP3K8

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 8 , AURA2 , COT , EST , ESTF , MEKK8 , TPL2 , Tpl-2 , c-COT

Cellular Localization

Cytoplasm

Background

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-MAP3K8 antibody [HL2118] used in IHC (Paraffin sections) (IHC-P). GTX638090

GTX638090 IHC-P Image

MAP3K8 antibody [HL2118] detects MAP3K8 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
MAP3K8 stained by MAP3K8 antibody [HL2118] (GTX638090) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP3K8 antibody [HL2118] used in Western Blot (WB). GTX638090

GTX638090 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (GTX638090) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP3K8 antibody [HL2118] used in Western Blot (WB). GTX638090

GTX638090 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (GTX638090) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations