Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8(GTX102711) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8(COT)(GTX102711) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.