GeneTex
United States (US)

MAP3K8 antibody [N3C3]

Overexpression
GTX102711_40073_20150226_IHC_M_19011506_916.jpg
GTX102711_WB_19011506_728.jpg
GTX102711_40073_20180914_WB_B_19011506_761.jpg
GTX102711_40073_IFA_19011506_917.jpg
GTX102711_IHC_19011506_341.jpg

Cat No. GTX102711

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase Kinase 8,Aura2,Cot,Est,Estf,Mekk8,Tpl2,Tpl-2,C-Cot,Map3K8

Background

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102711_40073_20150226_IHC_M_19011506_916.jpg

GTX102711 IHC-P Image

MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102711_WB_19011506_728.jpg

GTX102711 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX102711 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102711_40073_20180914_WB_B_19011506_761.jpg

GTX102711 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102711_40073_IFA_19011506_917.jpg

GTX102711 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8(GTX102711) antibody at 1:200 dilution.

GTX102711_IHC_19011506_341.jpg

GTX102711 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8(COT)(GTX102711) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169