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MAP3K8 antibody [N3C3]

Orthogonal Overexpression
Anti-MAP3K8 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102711
Anti-MAP3K8 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102711
Anti-MAP3K8 antibody [N3C3] used in Western Blot (WB). GTX102711
Anti-MAP3K8 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102711
Anti-MAP3K8 antibody [N3C3] used in Western Blot (WB). GTX102711

Cat. No. GTX102711

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MAP3K8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Lovo

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAP3K8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950870

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 8 , AURA2 , COT , EST , ESTF , MEKK8 , TPL2 , Tpl-2 , c-COT

Background

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAP3K8 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102711

GTX102711 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MAP3K8(GTX102711) antibody at 1:200 dilution.

Anti-MAP3K8 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102711

GTX102711 IHC-P Image

MAP3K8 antibody [N3C3] detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAP3K8 antibody [N3C3] used in Western Blot (WB). GTX102711

GTX102711 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP3K8 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102711

GTX102711 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8(COT)(GTX102711) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MAP3K8 antibody [N3C3] used in Western Blot (WB). GTX102711

GTX102711 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [N3C3] (GTX102711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Peterson AF et al. Cell Syst 2022; 13 (11) : 885-894.e4 Systematic analysis of the MAPK signaling network reveals MAP3K-driven control of cell fate.
Liu Y et al. Mol Endocrinol 2015; 29 (5) : 703-15 mitogen-activated protein kinase 8 (map3k8) mediates the signaling pathway of * stimulating progesterone production through g protein-coupled receptor 30 (gpr30) in mouse corpus luteum.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169