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MAP3K8 antibody

Anti-MAP3K8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03357
Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357
Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357
Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357
Anti-MAP3K8 antibody used in Flow cytometry (FACS). GTX03357
Anti-MAP3K8 antibody used in Western Blot (WB). GTX03357
Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357
Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

Cat. No. GTX03357

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($429)
See all MAP3K8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 5μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells

Note :

IHC-P
Antigen retrieval by Heat
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.05mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 8 , AURA2 , COT , EST , ESTF , MEKK8 , TPL2 , Tpl-2 , c-COT

Cellular Localization

Cytoplasm

Background

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-MAP3K8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03357

GTX03357 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX03357 MAP3K8 antibody.
Antigen retireval : enzyme antigen retrieval reagent for 15 min
Dilution : 5μg/mL

Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

GTX03357 IHC-P Image

IHC-P analysis of rat brain tissue using GTX03357 MAP3K8 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution).
Dilution : 1μg/ml

Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

GTX03357 IHC-P Image

IHC-P analysis of human pancreatic cancer tissue using GTX03357 MAP3K8 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution).
Dilution : 1μg/ml

Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

GTX03357 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX03357 MAP3K8 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution).
Dilution : 1μg/ml

Anti-MAP3K8 antibody used in Flow cytometry (FACS). GTX03357

GTX03357 FACS Image

FACS analysis of U937 cells using GTX03357 MAP3K8 antibody.
Antibody amount : 1μg/1x10⁶ cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-MAP3K8 antibody used in Western Blot (WB). GTX03357

GTX03357 WB Image

WB analysis of various sample lysates using GTX03357 MAP3K8 antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : Daudi whole cell lysate
Lane 7 : rat liver tissue lysate
Lane 8 : mouse liver tissue lysate
Loading : 50μg per lane
Dilution : 0.5 μg/mL_x000D_ _x000D_

Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

GTX03357 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX03357 MAP3K8 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution).
Dilution : 1μg/ml

Anti-MAP3K8 antibody used in IHC (Paraffin sections) (IHC-P). GTX03357

GTX03357 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX03357 MAP3K8 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution).
Dilution : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 429