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MAP4K3 antibody

Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745
Anti-MAP4K3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03745
Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745
Anti-MAP4K3 antibody used in Western Blot (WB). GTX03745
Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745

Cat. No. GTX03745

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($409)
See all MAP4K3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MAP4K3(Accession Q8IVH8), corresponding to amino acid residues M1-Q20.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase kinase 3 , GLK , MAPKKKK3 , MEKKK 3 , MEKKK3 , RAB8IPL1

Background

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Database

Research Area

DATA IMAGES

Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745

GTX03745 IHC-P Image

IHC-P analysis mouse kidney tissue using GTX03745 MAP4K3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-MAP4K3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03745

GTX03745 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX03745 MAP4K3 antibody.
Red : Primary antibody
Green : Beta-tublin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745

GTX03745 IHC-P Image

IHC-P analysis mouse brain tissue using GTX03745 MAP4K3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-MAP4K3 antibody used in Western Blot (WB). GTX03745

GTX03745 WB Image

WB analysis of HepG2 cell lysates using GTX03745 MAP4K3 antibody.

Anti-MAP4K3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03745

GTX03745 IHC-P Image

IHC-P analysis rat heart tissue using GTX03745 MAP4K3 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 409