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MAP4K4 antibody

Anti-MAP4K4 antibody used in Western Blot (WB). GTX116008
Anti-MAP4K4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116008
Anti-MAP4K4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116008
Anti-MAP4K4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116008

Cat. No. GTX116008

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MAP4K4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MAP4K4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase kinase 4 , FLH21957 , HEL-S-31 , HGK , MEKKK4 , NIK

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAP4K4 antibody used in Western Blot (WB). GTX116008

GTX116008 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP4K4 antibody (GTX116008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP4K4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116008

GTX116008 ICC/IF Image

MAP4K4 antibody detects MAP4K4 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP4K4 stained by MAP4K4 antibody (GTX116008) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-MAP4K4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116008

GTX116008 ICC/IF Image

MAP4K4 antibody detects MAP4K4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP4K4 protein stained by MAP4K4 antibody (GTX116008) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-MAP4K4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116008

GTX116008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SNU1 xenograft, using MAP4K4(GTX116008) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Hsu YH et al. Cancer Res 2014; Definition of PKC-α, CDK6 and MET as therapeutic targets in triple-negative breast cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169