GeneTex
United States (US)

MAPK4 antibody [N1C1]

GTX104128_IFA_19011506_894.jpg
GTX104128_39953_IHC_19011506_538.jpg
GTX104128_WB_19011506_222.jpg

Cat No. GTX104128

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAPK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ERK3 antibody, Erk4 antibody, PRKM4 antibody, p63MAPK antibody, MAPK4 antibody, MAP kinase isoform p63 antibody, MAPK 4 antibody, Erk3-related antibody, p63-MAPK antibody, ERK-4 antibody, MAP kinase 4 antibody, extracellular signal-regulated kinase 4 antibody, mitogen-activated protein kinase 4 antibody

Background

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus where it phosphorylates nuclear targets. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104128_IFA_19011506_894.jpg

GTX104128 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPK4(GTX104128) antibody at 1:200 dilution.

GTX104128_39953_IHC_19011506_538.jpg

GTX104128 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAPK4(GTX104128) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104128_WB_19011506_222.jpg

GTX104128 WB Image

Sample(30 ug whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX104128 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169