GeneTex
United States (US)

MAPKAP Kinase 3 antibody [N2C3]

KO/KD
GTX105147_39974_20170601_WB_KO_watermark_19011506_423.jpg
GTX105147_39974_WB_M_spleen_19011506_779.jpg
GTX105147_WB_19011506_800.jpg
GTX105147_IFA_19011506_306.jpg

Cat No. GTX105147

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , mouse spleen

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAPKAP Kinase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3PK antibody, MAPKAP3 antibody, MAPKAPK3 antibody, MAPKAP kinase 3 antibody, MAP kinase-activated protein kinase 3 antibody, MAPK-activated protein kinase 3 antibody, chromosome 3p kinase antibody, mitogen-activated protein kinase-activated protein kinase 3 antibody

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. [provided by RefSeq]

Database

DATA IMAGES
GTX105147_39974_20170601_WB_KO_watermark_19011506_423.jpg

GTX105147 WB Image

KO/KD

Wild-type (WT) and MAPKAP Kinase 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAPKAP Kinase 3 antibody [N2C3] (GTX105147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX105147_39974_WB_M_spleen_19011506_779.jpg

GTX105147 WB Image

Sample (50 ug of whole cell lysate)
A: mouse spleen
10% SDS PAGE
GTX105147 diluted at 1:10000

GTX105147_WB_19011506_800.jpg

GTX105147 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
10% SDS PAGE
GTX105147 diluted at 1:3000

GTX105147_IFA_19011506_306.jpg

GTX105147 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPKAP Kinase 3 (GTX105147) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169