GeneTex

  • Country / Location Selection

United States (US)

MAPKAP Kinase 3 antibody [N2C3]

KO/KD
Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147
Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147
Anti-MAPKAP Kinase 3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105147
Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147

Cat. No. GTX105147

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all MAPKAP Kinase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse spleen , H1299 , HeLaS3 , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAPKAP Kinase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950884

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase-activated protein kinase 3 , 3PK , MAPKAP-K3 , MAPKAP3 , MAPKAPK-3 , MDPT3 , MK-3

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147

GTX105147 WB Image

KO/KD

Wild-type (WT) and MAPKAP Kinase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MAPKAP Kinase 3 antibody [N2C3] (GTX105147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147

GTX105147 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
10% SDS PAGE
GTX105147 diluted at 1:3000

Anti-MAPKAP Kinase 3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105147

GTX105147 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAPKAP Kinase 3 (GTX105147) antibody at 1:200 dilution.

Anti-MAPKAP Kinase 3 antibody [N2C3] used in Western Blot (WB). GTX105147

GTX105147 WB Image

Sample (50 ug of whole cell lysate)
A: mouse spleen
10% SDS PAGE
GTX105147 diluted at 1:10000

REFERENCE

There are currently no references for MAPKAP Kinase 3 antibody [N2C3] (GTX105147). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for MAPKAP Kinase 3 antibody [N2C3] (GTX105147). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169