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MAPKAPK-2 antibody

Orthogonal
Anti-MAPKAPK-2 antibody used in Western Blot (WB). GTX50617
Anti-MAPKAPK-2 antibody used in Western Blot (WB). GTX50617
Anti-MAPKAPK-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50617

Cat. No. GTX50617

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MAPKAPK-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.332~336 (P-Q-T-P-L) derived from human MAPKAPK-2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase-activated protein kinase 2 , MAPKAP-K2 , MK-2 , MK2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MAPKAPK-2 antibody used in Western Blot (WB). GTX50617

GTX50617 WB Image

Orthogonal

WB analysis of extracts from 293 cells using GTX50617 MAPKAPK-2 antibody with or without blocking peptide.

Anti-MAPKAPK-2 antibody used in Western Blot (WB). GTX50617

GTX50617 WB Image

Orthogonal

WB analysis of extracts from JK, 3T3, and C6 cells using GTX50617 MAPKAPK-2 antibody.

Anti-MAPKAPK-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50617

GTX50617 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed HeLa cells using GTX50617 MAPKAPK-2 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399