MAPKAPK-2 (phospho Thr334) antibody

Cat. No. GTX50321

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX50321 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200
IHC-P	1:50-1:100

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from human MAPKAPK-2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase-activated protein kinase 2 , MAPKAP-K2 , MK-2 , MK2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 9261 MAPKAPK2
UniProt: P49137 MAPKAPK2

Research Area

DATA IMAGES

GTX50321 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50321 MAPKAPK-2 (phospho Thr334) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

GTX50321 WB Image

WB analysis of extracts from HeLa cells untreated (lane 1) or treated with UV (lane 2) using GTX50321 MAPKAPK-2 (phospho Thr334) antibody.

GTX50321 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50321 MAPKAPK-2 (phospho Thr334) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty