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MARCH10 antibody, Internal

Anti-MARCH10 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88596
Anti-MARCH10 antibody, Internal used in Western Blot (WB). GTX88596

Cat. No. GTX88596

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all MARCH10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88596-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane associated ring-CH-type finger 10 , MARCH-X , RNF190

Background

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

Database

DATA IMAGES

Anti-MARCH10 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88596

GTX88596 IHC-P Image

IHC-P analysis of human testis using GTX88596 MARCH10 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-MARCH10 antibody, Internal used in Western Blot (WB). GTX88596

GTX88596 WB Image

WB analysis of rat testis lysate using GTX88596 MARCH10 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399