MARCH2 antibody

Cat. No. GTX116151

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($419), 25 μl ($169)

See all MARCH2 products

Datasheet
Document

View product citations for antibody GTX116151 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000

Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2 membrane

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MARCH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane associated ring-CH-type finger 2 , HSPC240 , MARCH-II , RNF172

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Lysosome membrane; Multi-pass membrane protein , Endosome membrane; Multi-pass membrane protein

Background

MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM]

Database

Gene ID: 51257 MARCH2
UniProt: Q9P0N8 MARCH2

Research Area

DATA IMAGES

GTX116151 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2 membrane
12% SDS PAGE
GTX116151 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 419

25 μl

$ 169

Qty