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MARCH5 antibody

Anti-MARCH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03436
Anti-MARCH5 antibody used in Western Blot (WB). GTX03436

Cat. No. GTX03436

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all MARCH5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Predict Reactivity

Bovine, Chicken, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

membrane associated ring-CH-type finger 5 , MARCH-V , MITOL , RNF153

Cellular Localization

Mitochondrion outer membrane , Endoplasmic reticulum membrane

Background

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-MARCH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03436

GTX03436 IHC-P Image

IHC-P analysis of human heart tissue using GTX03436 MARCH5 antibody.
Antigen retrieval : Heat mediation with a EDTA buffer (pH9.0)
Dilution : 1:500

Anti-MARCH5 antibody used in Western Blot (WB). GTX03436

GTX03436 WB Image

WB analysis of various sample lysates using GTX03436 MARCH5 antibody.
Lane 1 : U-251 MG whole cell lysate
Lane 2 : T47D whole cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Mouse kidney tissue lysate
Dilution : 1:1000
Loading : 20 μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 399