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MARCKS antibody

Anti-MARCKS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50602
Anti-MARCKS antibody used in Western Blot (WB). GTX50602

Cat No. GTX50602

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.156~160 (R-F-S-F-K) derived from human MARCKS.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50602

GTX50602 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50602 MARCKS antibody.

Anti-MARCKS antibody used in Western Blot (WB). GTX50602

GTX50602 WB Image

WB analysis of extracts from 3T3 cells using GTX50602 MARCKS antibody with or without blocking peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf