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MARCKS (phospho Ser152/Ser156) antibody

Anti-MARCKS (phospho Ser152/Ser156) antibody used in Western Blot (WB). GTX17544

Cat. No. GTX17544

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Chicken, Xenopus
Package
50 μl ($399)
See all MARCKS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000

Note :

WB
Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase

PROPERTIES

Form

Liquid

Buffer

10mM HEPES pH7.5, 0.15M NaCl, 0.1mg/ml BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer152,156 of MARCKS.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS (phospho Ser152/Ser156) antibody used in Western Blot (WB). GTX17544

GTX17544 WB Image

WB analysis of lambda-phosphatase treated or un-treated rat brain tissue lysate using GTX17544 MARCKS (phospho Ser152/Ser156) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 399