MARCKS (phospho Ser152) antibody

Cat. No. GTX50312

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse
Package	100 μl ($399)

See all MARCKS products

Datasheet
Document

View product citations for antibody GTX50312 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:100-1:200

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is raised against phopsho peptide of human MARCKS Ser158, which shares 100% identity identity with rat MARCKS around Ser152.

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from human MARCKS.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4082 MARCKS
UniProt: P29966 MARCKS

Research Area

DATA IMAGES

GTX50312 WB Image

WB analysis of extracts from 3T3 cells using GTX50312 MARCKS (phospho Ser152) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

GTX50312 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50312 MARCKS (phospho Ser152) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty