GeneTex
  • Country / Location Selection

United States (US)

MARCKS (phospho Ser158) antibody

Anti-MARCKS (phospho Ser158) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50312
Anti-MARCKS (phospho Ser158) antibody used in Western Blot (WB). GTX50312

Cat No. GTX50312

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 158 (R-F-S(p)-F-K) derived from human MARCKS.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS (phospho Ser158) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50312

GTX50312 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50312 MARCKS (phospho Ser158) antibody.

Anti-MARCKS (phospho Ser158) antibody used in Western Blot (WB). GTX50312

GTX50312 WB Image

WB analysis of extracts from 3T3 cells using GTX50312 MARCKS (phospho Ser158) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for MARCKS (phospho Ser158) antibody (GTX50312). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for MARCKS (phospho Ser158) antibody (GTX50312). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf