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MARCKS (phospho Ser156) antibody

Anti-MARCKS (phospho Ser156) antibody used in Western Blot (WB). GTX50290
Anti-MARCKS (phospho Ser156) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50290

Cat. No. GTX50290

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all MARCKS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is raised against phopsho peptide of human MARCKS Ser162, which shares 100% identity identity with rat MARCKS around Ser156.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 162 (K-K-S(p)-F-K) derived from human MARCKS.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS (phospho Ser156) antibody used in Western Blot (WB). GTX50290

GTX50290 WB Image

WB analysis of extracts from 293 cells untreated or treated with PMA using GTX50290 MARCKS (phospho Ser156) antibody.

Anti-MARCKS (phospho Ser156) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50290

GTX50290 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50290 MARCKS (phospho Ser156) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399