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MARCKS (phospho Ser156) antibody

Anti-MARCKS (phospho Ser156) antibody used in Western Blot (WB). GTX32212

Cat. No. GTX32212

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all MARCKS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

This antibody is raised against phopsho peptide of human MARCKS Ser162, which shares 100% identity identity with rat MARCKS around Ser156.

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of MARCKS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

myristoylated alanine rich protein kinase C substrate , 80K-L , MACS , PKCSL , PRKCSL

Cellular Localization

Cytoplasm, cytoskeleton,Membrane

Background

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MARCKS (phospho Ser156) antibody used in Western Blot (WB). GTX32212

GTX32212 WB Image

WB analysis of PMA-treated MCF7 (A), PMA-treated SP2/0 (B),PMA-treated PC12 (C) whole cell lysates using GTX32212 MARCKS (phospho Ser156) antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399