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MARK2 antibody [GT1279]

Recombinant antibody
Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191
Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191
Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191

Cat. No. GTX03191

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1279

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all MARK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Observed MW

82/78 kDa. The observed M.W. is based on the following publication. PMID: 23326495

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human MARK2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule affinity regulating kinase 2 , EMK-1 , EMK1 , PAR-1 , Par-1b , Par1b

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton , Cell projection, dendrite

Background

This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]

Database

Research Area

DATA IMAGES

Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191

GTX03191 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARK2 antibody [GT1279] (GTX03191) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 75 kDa, and 80 kDa kDa. It is possibly due to alternative splicing.
Reference: PMID: 23326495

Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191

GTX03191 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARK2 antibody [GT1279] (GTX03191) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MARK2 antibody [GT1279] used in Western Blot (WB). GTX03191

GTX03191 WB Image

WB analysis of various samples using GTX03191 MARK2 antibody [GT1279].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439